Significance of the expression of EMMPRIN, CD24 and p16INK4a promoter methylation in gastric carcinoma

Introduction: A lot of effort has been made to investigate the prognostic biomarkers, including epigenetic markers, in cancers, helping to identify high-risk cancer patients who might need adjuvant treatment after surgery. Studies suggest complex genetic and epigenetic profiles of the gastric carcinoma patients, indicating the need for the identification of molecular markers for this tumour type. Aim: The aim of the study was to clarify the role of extracellular matrix metalloproteinase inducer (EMMPRIN) (CD147), CD24 and methylation of p16 promoter in the genesis and progression of gastric carcinoma. Materials and methods: Expression of EMMPRIN (CD147) and CD24 was examined immunohistochemically in tissue sections from gastric carcinoma (n=80) and noncancerous gastric cases [chronic gastritis with intestinal metaplasia (n=16) and gastric dysplasia (n=20)]. In addition, 16 cases of apparently normal gastric were used as control and the expression was compared with clinicopathological parameters of cancer cases, including 5-year survival. The methylation frequency of the p16INK4a promoter was determined by methylation-specific PCR. Results: Both CD147 and CD24 were gradually increased from normal mucosa to carcinomas through metaplastic and dysplastic mucosa of the stomach (P0.05). Interestingly, there was a significantly positive relationship between their expression and 5-year survival (P
Source: Egyptian Journal of Pathology - Category: Pathology Tags: Original Articles Source Type: research