Targeting Human Long Noncoding Transcripts by Endoribonuclease-Prepared siRNAs

We present a compendium of lncRNA expression data for 11 human cancer cell lines. Furthermore, we show that the resource is suitable for combined knockdown and localization analysis. We discuss challenges in sequence annotation of lncRNAs with respect to their often low and cell type–specific expression and specify esiRNAs that are suitable for targeting lncRNAs in commonly used human cell lines.

Source: Journal of Biomolecular Screening - August 19, 2015 Category: Molecular Biology Authors: Theis, M., Paszkowski-Rogacz, M., Weisswange, I., Chakraborty, D., Buchholz, F. Tags: Articles Source Type: research