Acoustic Liquid Handling for Rapid siRNA Transfection Optimization

In this study, we describe a methodology to utilize the flexibility and low-volume range of the Echo acoustic liquid handler to rapidly screen a matrix of transfection conditions. The matrix includes six different transfection lipids from three separate vendors across a broad range of concentrations. Our results validate acoustic liquid transfer for the delivery of siRNAs and transfection reagents. Finally, this methodology is applied to rapidly optimize transfection conditions across many tissue culture cell lines derived from various originating tissues.
Source: Journal of Biomolecular Screening - Category: Molecular Biology Authors: Tags: Articles Source Type: research