Elutriation for Cell Cycle Synchronization in Fission Yeast

Cell synchronization is a powerful technique for studying the eukaryotic cell cycle events precisely. The fission yeast is a rod-shaped cell whose growth is coordinated with the cell cycle. Monitoring the cellular growth of fission yeast is a relatively simple way to measure the cell cycle stage of a cell. Here, we describe a detailed method of unperturbed cell synchronization, named centrifugal elutriation, for fission yeast.

Source: Springer protocols feed by Cell Biology - August 10, 2015 Category: Cytology Source Type: news