Detection of Protein–Protein Interactions in Tobacco BY-2 Cells Using Bimolecular Fluorescence Complementation

Knowledge of protein–protein interactions in the plant cell is invaluable for furthering our understanding of the functions of these proteins. Many of the methods available for the study of these interactions, such as yeast two-hybrid and co-immunoprecipitation assays, rely on in vitro techniques. Here we describe the use of bimolecular fluorescence complementation for the study of protein–protein interactions in vivo, using simple techniques and accessible materials.

Source: Springer protocols feed by Cell Biology - August 10, 2015 Category: Cytology Source Type: news