Determination of Cell Uptake Pathways for Tumor Inhibitor Lysyl Oxidase Propeptide

The lysyl oxidase propeptide (LOX-PP) is derived from pro-lysyl oxidase (Pro-LOX) by extracellular biosynthetic proteolysis. LOX-PP inhibits breast and prostate cancer xenograft tumor growth and has tumor suppressor activity. Although, several intracellular targets and molecular mechanisms of action of LOX-PP have been identified, LOX-PP uptake pathways have not been reported. Here we demonstrate that the major uptake pathway for recombinant LOX-PP (rLOX-PP) is PI3K-dependent macropinocytosis in PWR-1E, PC3, SCC9, MDA-MB-231 cell lines.

Source: Molecular Oncology - August 5, 2015 Category: Cancer & Oncology Authors: Gokhan Baris Ozdener, Manish V. Bais, Philip C. Trackman Source Type: research