Proteomic Profiling of Hyperplasia/Atypia and Adenoma-induced by NNK in Mouse Lung identified Multiple Proteins as Potential Biomarkers for Early Detection

Publication date: Available online 3 August 2015 Source:EuPA Open Proteomics Author(s): Arunangshu Das, James D. Bortner, Cesar Aliaga, Timothy Cooper, Anne Stanley, Bruce A. Stanley, Chandra P. Belani, Karam El-Bayoumy We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g. Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research