RAC1 regulate tumor necrosis factor‐α‐mediated impaired osteogenic differentiation of dental pulp stem cells
In this study we analyze the function of TNF‐α (100 ng/mL) on osteogenic differentiation of human DPSCs for the first time and identify the underlying molecule mechanisms. Our data revealed that TNF‐α with higher concentration significantly reduced mineralization and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP) and runt‐related transcription factor 2 (RUNX2). Further, we revealed that TNF‐α could suppress the osteogenic differentiation of DPSCs via increasing the expression of RAC1, which could activate the Wnt/β‐catenin signaling pathway and liberate β‐catenin to translocate into the nucleus. Genetic silencing of RAC1 expression using siRNA restored osteogenic differentiation of DPSCs. Our findings may provide a potential approach to bone regeneration in inflammatory microenvironments.
TNF‐α could suppress the osteogenic differentiation of DPSCs via increasing the expression of RAC1, which could activate the Wnt/β‐catenin signaling pathway and liberate β‐catenin to translocate into the nucleus. Genetic silencing of RAC1 expression using siRNA restored osteogenic differentiation of DPSCs.
Source: Development, Growth and Differentiation - Category: Research Authors: Guijuan Feng, Qijie Shen, Min Lian, Zhifeng Gu, Jing Xing, Xiaohui Lu, Dan Huang, Liren Li, Shen Huang, Yi Wang, Jinlong Zhang, Jiahai Shi, Dongmei Zhang, Xingmei Feng Tags: Original Article Source Type: research