The preparation and culture of washed human sperm: A comparison of a suite of protein-free media with media containing human serum albumin

Conclusion These results support the use of PF media supplemented with methylcellulose as an alternative to HSA, although a modification to the manufacturer's protocol of 6-min pre-incubation before assessing sperm motility must be used. Further studies should investigate the function of such sperm prepared in PF medium.
Source: Asian Pacific Journal of Reproduction - Category: Reproduction Medicine Source Type: research