COX-2 gene promoter DNA methylation status in eutopic and ectopic endometrium of Egyptian women with endometriosis

Background: The pathophysiology of COX-2 expression in endometriosis is a matter of debate.Aim: To investigate the role of DNA methylation of NF-IL6 site within the promoter of COX-2 gene in the pathogenesis of endometriosis.Methods: The endometrial tissues (ectopic and eutopic) were collected from 60 women with endometriosis and 30 women without endometriosis (control group). The methylation status of COX-2 was examined by methylation specific PCR. Quantitative real-time PCR (RT-PCR) was performed to measure COX-2 mRNA level in endometrial tissues.

Source: Journal of Reproductive Immunology - July 18, 2015 Category: Allergy & Immunology Authors: Haidy E Zidan, Noha A Rezk, Amr Abd Almohsen Alnemr, Amany M. Abd el Ghany Source Type: research