A Quality Control of Proteomic Experiments Based on Multiple Isotopologous Internal Standards

Publication date: Available online 13 July 2015 Source:EuPA Open Proteomics Author(s): Adele Bourmaud , Sebastien Gallien , Bruno Domon The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications. Graphical abstract

Source: EuPA Open Proteomics - July 13, 2015 Category: Bioinformatics Source Type: research