Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides

Conclusions: This paper comprises an assay toolbox for identification and characterization of the synthetases and nucleases regulating cellular 2-5A levels. Assays are presented for both enzyme families. The assays can also be used to address a broader cellular role of the OAS enzymes, based on the multiple substrate specificity intrinsic to these proteins.
Source: BMC Biochemistry - Category: Biochemistry Authors: Source Type: research
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