Oxidative and proteolysis‐related parameters of skeletal muscle from hamsters with experimental pulmonary emphysema: a comparison between papain and elastase induction

The objective of this study was to investigate whether emphysema induced by elastase or papain triggers the same effects on skeletal muscle, related to oxidative stress and proteolysis, in hamsters. For this purpose, we evaluated pulmonary lesions, body weight, muscle loss, oxidative stress (thiobarbituric acid‐reactive substances, total and oxidized glutathiones, chemiluminescence stimulated by tert‐butyl hydroperoxide and carbonyl proteins), chymotrypsin‐like and calpain‐like proteolytic activities and muscle fibre cross‐sectional area in the gastrocnemius muscles of emphysemic hamsters. Two groups of animals received different intratracheal inductions of experimental emphysema: by 40 mg/ml papain (EP) or 5.2 IU/100 g animal (EE) elastase (n = 10 animals/group). The control group received intratracheal instillation of 300 μl sterile NaCl 0.9%. Compared with the control group, the EP group had reduced muscle weight (18.34%) and the EE group had increased muscle weight (8.37%). Additionally, tert‐butyl hydroperoxide‐initiated chemiluminescence, carbonylated proteins and chymotrypsin‐like proteolytic activity were all elevated in the EP group compared to the CS group, while total glutathione was decreased compared to the EE group. The EE group showed more fibres with increased cross‐sectional areas and increased calpain‐like activity. Together, these data show that elastase and papain, when used to induce experimental models of emphysema, lead to dif...
Source: International Journal of Experimental Pathology - Category: Pathology Authors: Tags: Original Article Source Type: research