Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases

We describe an example of a recruitment assay using the GBD of PAK1 to monitor Rac1 activity and explain how the assay can be expanded to determine the subcellular localization of activation of other GTPases.

Source: Springer protocols feed by Cell Biology - January 29, 2014 Category: Cytology Source Type: news