Inter-laboratory Evaluation of Instrument Platforms and Experimental Workflows for Quantitative Accuracy and Reproducibility Assessment

Publication date: Available online 11 June 2015 Source:EuPA Open Proteomics Author(s): Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesco Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Miguel Marcilla , Mathew J. McKay , Mark P. Molloy , Leanne B. Ohlund , Young-Ki Paik , Alberto Paradela , Mark Raftery , Eduard Sabidó , Lekha Sleno , Daniel Wilffert , Justina C. Wolters , Jong Shin Yoo , Victor Zgoda , Carol E. Parker , Christoph H. Borchers The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC-MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC-MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research