Detection and Quantification of Allosteric Modulation of Endogenous M4 Muscarinic Acetylcholine Receptor Using Impedance-Based Label-Free Technology in a Neuronal Cell Line

In this study, we assessed the ability of an impedance-based label-free technology, xCELLigence, to detect allosteric modulation in a neuronal cell line natively expressing rodent M4 muscarinic acetylcholine receptors. We were able to demonstrate that positive allosteric modulation of the endogenous M4 muscarinic acetylcholine receptor can be detected using this technology. Importantly, the allosteric parameters estimated from the label-free approach are comparable to those estimated from endpoint-based assays.

Source: Journal of Biomolecular Screening - May 19, 2015 Category: Molecular Biology Authors: Chen, A. N. Y., Malone, D. T., Pabreja, K., Sexton, P. M., Christopoulos, A., Canals, M. Tags: Original Research Source Type: research