Quantitative analysis of the erythrocyte membrane proteins in polycythemia vera patients treated with hydroxycarbamide

Publication date: Available online 5 May 2015 Source:EuPA Open Proteomics Author(s): Darshana Kottahachchi , Lallindra Gooneratne , Anil Jayasekera , Dorota Muth-Pawlak , Robert Moulder , Susumu Y. Imanishi , Ari Ariyaratne , Anne Rokka , Garry L. Corthals More than 90% of polycythemia vera (PV) patients have a mutation in the protein JAK2, which is closely associated with the erythrocyte membrane. With the comparison of 1-D gels of erythrocyte membranes obtained from PV patients treated with hydroxycarbamide and those of untreated controls we observed significant differences in the region of 40–55kDa. On the basis of the LC–MS/MS analysis of this region we report up-regulation of four protein disulfide isomerases, which was subsequently confirmed by targeted mass spectrometric analysis. In further studies it will be prudent to compare this in patients both treated and not treated with hydroxycarbamide. Graphical abstract

Source: EuPA Open Proteomics - May 16, 2015 Category: Bioinformatics Source Type: research