A novel HAC1-based Dual-Luciferase Reporter Vector for detecting endoplasmic reticulum stress and unfolded protein response in yeast Saccharomyces cerevisiae

In this study, we developed a dual-luciferase gene reporter system to monitor UPR activation in live cells of the yeast Saccharomyces cerevisiae by taking advantage of the HAC1 intron and its unconventional splicing-regulation mechanism. We showed that this reporter can be used to monitor UPR in live cells with high sensitivity.
Source: Plasmid - Category: Biotechnology Source Type: research