Poly-3-hydroxybutyrate-co-3-hydroxyvalerate containing scaffolds and their integration with osteoblasts as a model for bone tissue engineering

Nano/micro engineered polymeric materials offer expansive scope of biomimetic scaffolds for bone tissue engineering especially those involving electrospun biodegradable nanofibers incorporated with inorganic nanoparticles, thus mimicking the extracellular matrix of bone both structurally and chemically. For the first time, poly-3-hydroxybutyrate-co-3-hydroxyvalerate containing natural poly-(α, β)-DL-aspartic acid and inorganic hydroxyapatite nanofibers were fabricated using poly-3-hydroxybutyrate-co-3-hydroxyvalerate: poly-(α, β)-DL-aspartic acid at a ratio of 80:20 (w/w) added with 1% (w/v) of hydroxyapatite, by the process of electrospinning. The surface morphology, chemical, and mechanical properties of electrospun poly-3-hydroxybutyrate-co-3-hydroxyvalerate, poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid, and poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid/hydroxyapatite nanofibers were characterized by using field emission scanning electron microscope, Fourier transform infrared spectroscopy, and tensile tester, respectively. Human fetal osteoblasts were cultured on different nanofibrous scaffolds for evaluating the cell proliferation, alkaline phosphatase activity, and mineralization. Cells on poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid/hydroxyapatite scaffolds demonstrated higher proliferation (30.10%) and mineral deposition (37.60%) th...
Source: Journal of Biomaterials Applications - Category: Materials Science Authors: Tags: Hard Tissues and Materials Source Type: research