Maintenance and genetic load of plasmid pKON1 of Paracoccus kondratievae, containing a highly efficient toxin–antitoxin module of the hipAB family

Publication date: Available online 6 March 2015 Source:Plasmid Author(s): Jakub Czarnecki , Lukasz Dziewit , Lukasz Kowalski , Magdalena Ochnio , Dariusz Bartosik Paracoccus kondratievae NCIMB 13773T, isolated from the maize rhizosphere, carries a large (95,049 bp) plasmid pKON1, whose structure has been significantly influenced by transposition. Almost 30% of the plasmid genome is composed of complete or truncated insertion sequences (ISs), representing seven IS families. The ISs are accompanied by numerous genes and gene clusters commonly found in bacterial chromosomes, encoding, among others, (i) a putative type III secretion system of the Rhizobiales-T3SS family, (ii) a type I restriction–modification system associated with the anti-codon nuclease (ACNase) gene prrC and (iii) OstA and OstB proteins involved in trehalose synthesis. The backbone of pKON1 is composed of replication and partitioning modules conserved in several large alphaproteobacterial replicons, including secondary chromid pAMI6 of Paracoccus aminophilus JCM 7686 and chromosome 2 (chromid) of Rhodobacter sphaeroides 2.4.1. pKON1 also contains a toxin–antitoxin system of the hipAB family, whose presence precludes removal of the plasmid from bacterial cells. This system, unlike two other related hipAB-family loci originating from plasmid pAMI8 and the chromosome of Paracoccus aminophilus JCM 7686, is highly efficient and permits very stable maintenance of a heterologous replicon in various ho...

Source: Plasmid - April 11, 2015 Category: Biotechnology Source Type: research