pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) Using Amphoteric Azo Dyes

Publication date: Available online 9 April 2015 Source:EuPA Open Proteomics Author(s): Marina Hanneken , Karel Šlais , Simone König Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal Comparative Fluorescence Gel Electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the y-direction. However, control of the first dimension, pI (x-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit. Graphical abstract

Source: EuPA Open Proteomics - April 9, 2015 Category: Bioinformatics Source Type: research