Use of proteasome inhibitors.

Use of proteasome inhibitors. Curr Protoc Immunol. 2015;109:9.10.1-9 Authors: Downey SL, Florea BI, Overkleeft HS, Kisselev AF Abstract Proteasome inhibitors are indispensable research tools in immunology and cell biology. With numerous proteasome inhibitors available commercially, choosing the appropriate compound for a biological experiment may be challenging, especially for a novice. This unit provides an overview of the proteasome inhibitors commonly used in research. It discusses how to select an appropriate highly specific inhibitor, its concentration, and length of exposure for mammalian cell culture experiments. In addition, assays that can be used to confirm proteasome inhibition are discussed. © 2015 by John Wiley & Sons, Inc. PMID: 25845565 [PubMed - in process]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research